This technology is needed to de-
monstrate the effectiveness of bio-
remediation and for demonstra-
tion of additional bioremediation benefits from other techniques. The various advanced monitoring tech-
niques developed and applied as part of this task (DNAPLs probe analysis, lipid analysis, activity, and biomass measurements) all contribute to documenting the ne-
cessary changes in microbial pop-
ulations. In addition, these tech-
niques afford the opportunity to give feedback during the remedia-
tion operation so that procedures may be changed to increase the effectiveness of the remediation.

Accomplishments

New DNAPLs isolation and pre-
servation methods were develop-
ed and used during the program (based on ion exchange resins) to allow for long-term archiving of site DNAPLs for future analysis. DNAPLs probes were successfully used to detect the densities of specific functional groups impor-
tant to the bioremediation process at the Savannah River Site. PLFA analyses were used to indicate the activity of the organisms present. FA tests provided key information about microbial com-
munity structure and function.